First, boot

Remove the desiccant from the sample compartment before starting up and check that the power supply is connected. Turn on the power switch of the instrument, wait for the instrument to pass the self-test, and prohibit opening the sample chamber during the self-test.

2. After the self-test of the instrument is used (OK appears in 7 self-test items), press the [MAIN MENU] key (main menu). The screen displays the following five function items: 1. Phtometry (Quantitative operation); 2. Wavelength Scan (wavelength scan mode); 3. Time Scan (time curve scan); 4. System (system correction); 5. Data display (photometric direct measurement mode). Press the number key before the corresponding option to enter the next submenu of this option.

1. Phtometry

1) Press the [MAIN MENU] key, then press the [1] key to enter the Phtometry sub menu, select the corresponding number to select the desired measurement method: 1% T/ABS (transmittance/absorbance measurement mode); 2Ratio (Proportional mode); 3Concentration (concentration mode or standard curve mode);

2) Press the number [1] key to enter the %T/ABS submenu. Select the corresponding number key to set the measurement condition: 1 NUM WL (Set the number of test wavelengths, up to 6 Different wavelengths); 2WL Setting (Specify the test wavelength specific value) 3Data Mode (Select absorbance or transmittance measurement), after setting, click [Enter] to confirm, after all the items are set, press number [0] OK, wait for the instrument to adjust to the ready state, AUTOZERO appears on the screen, put the cuvette containing the blank solution into the sample chamber, press [Start/Stop] key to automatically adjust the zero point, after the auto-zeroing is completed, Place the sample in the light path and press the [Start/Stop] key to make a measurement. The instrument's display automatically gives the corresponding wavelength value.

3) Press the number [3] key to enter 3Concentration (concentration measurement mode or standard curve mode), select the corresponding number to set the conditions (wavelength number, wavelength value, standard solution number and concentration), and click [Enter] after setting Press key to confirm. After all the items are set, press the number [0] key to confirm. Wait for the instrument to adjust to the ready state. AUTOZERO appears on the screen. Place the cuvette containing the blank solution in the sample room and press [Start/ Stop button is used to perform zero point auto-adjustment. After auto-zero is completed, the standard solution is placed in the light path. Press the [Start/Stop] key in order from the lowest to the highest concentration to measure. After the measurement is completed, the instrument will automatically give the standard. Curves, there are three options below the standard curve: 1 Process (change the coordinates of the standard curve and the data of the observed concentration regression curve); 2Measure (measurement directly into the sample); 3Print (print the concentration regression curve spectrum and data), select the corresponding The number can perform the corresponding function.
4) If you wish to return to the previous menu, press the [CLEAR RETURN] button to return. Return to the main menu and press the [MAIN MENU] button.
2. Wavelength Scan (wavelength scan mode)

1) Parameter modification: Press the [MAIN MENU] key, then press the [2] key to enter the 2 Wavelength Scan submenu, select the corresponding digit to select the desired modification, and modify the starting wavelength of the scan. Measurement mode, upper and lower limits of map coordinates, scan speed, and more. After the modification is completed, press the number [0] key to confirm.

2) Wavelength scanning: Place two cuvettes with blank solution and sample solution, respectively, into the colorimetric tank, and press [Start/Stop] key to scan the spectrum. (If you want to stop scanning, press [Start/Stop again) [] key), until the instrument automatically baseline correction, prompted to pull into the sample automatically test, after the completion of the test scan spectrum appears, below the corresponding data processing options 1Process2Baseline3Print;

3) Data processing: Press the number [1] key to enter 1Process (data processing), you can read the peaks and valleys, modify the coordinates, display all the data, find the first order countdown and so on.

3. Time Scan Same as above (2) operation.

4.System (system correction), generally do not do.

5.Data display (photometric direct measurement mode) as above (two) operation.

Third, shut down

Invert the solution in the cuvette, then rinse the cuvette with distilled water or organic solvent to clean; turn off the power Put the desiccant into the sample chamber, put on the dust cover, and use the registration to get approval from the management teacher. go away.

Precautions:

1. Remove the desiccant from the sample chamber before starting up. Do not open the sample chamber cover during the self-test.
2. The solution in the cuvette is preferably 2/3 to 4/5 of the dish height, and should not be overfilled to prevent the liquid from overflowing and corroding the instrument. The cuvette should be kept clean during the measurement, and the liquid droplets on the pool wall should be wiped dry with the lens tissue. Do not pinch the light surface with your hands. When measuring ultraviolet wavelengths, quartz cuvettes are used.
3. During the measurement, it is forbidden to place the reagent or liquid substance on the surface of the instrument. If there is solution overflow or other reasons, the sample tank is dirty, and it should be cleaned as soon as possible.

4. After the end of the experiment, the solution in the cuvette is depleted, and then the cuvette is washed with distilled water or an organic solvent to clean it and dry it upside down. Turn off the power and put the desiccant into the sample chamber, put on the dust cover, make registration, and get approval from the management teacher before leaving.

Problem processing:
1. If the instrument fails to initialize, shut down and restart. If it is not successful, please report to the management teacher.
2. If the absorption value is abnormal, check in sequence: whether the wavelength setting is correct (re-adjust the wavelength and re-zero), whether the measurement is zero (if mis-operated, re-zero), and whether the cuvette is used wrongly (measurement of ultraviolet In the case of a band, quartz cuvettes are used, and sample preparation is erroneous (in case of errors, prepare the sample again).

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